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M9490129.TXT
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1994-09-03
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Document 0129
DOCN M9490129
TI [Studies of the resistance of the human immunodeficiency virus to
azidothymidine. I. Kinetic studies of the interaction between reverse
transcriptase and a series of its mutant forms with substrates and
azidothymidine-5'-trisphosphate]
DT 9411
AU Pokholok DK; Gudima SO; Esipov DS; Dobrynin VN; Memelova LV; Rechinskii
VO; Kochetkov SN
SO Biokhimiia. 1994 May;59(5):739-47. Unique Identifier : AIDSLINE
MED/94318764
AB Prolonged therapy of AIDS patients with azidothymidine results in the
development of resistance to the drug. This phenomenon is accompanied by
the appearance of point mutations in the pol gene coding for reverse
transcriptase (RT). Kinetic studies of interactions of wild type RT and
its forms containing the above-mentioned mutations with substrates and
azidothymidine 5'-triphosphate have been carried out. Considerable
differences in the affinities of RT and the mutants for RNA and DNA
heteropolymer templates were established. The mutations did not affect
the RT affinity for dNTP; however, its location influenced considerably
the inhibition of the reaction with azidothymidine 5'-triphosphate.
DE Amino Acid Sequence Base Sequence Drug Resistance, Microbial/GENETICS
DNA, Viral/METABOLISM English Abstract Genes, pol Human HIV-1/*DRUG
EFFECTS/ENZYMOLOGY Kinetics Molecular Sequence Data Point Mutation
Reverse Transcriptase/GENETICS/*METABOLISM RNA, Viral/METABOLISM
Substrate Specificity Thymine Nucleotides/*METABOLISM
Zidovudine/*ANALOGS & DERIVATIVES/METABOLISM/*PHARMACOLOGY JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).